Overview | PrinterFriendlyVersion |
Ex/Em(nm) | None/None |
MW | 307.34 |
CAS# | N/A |
Solvent | N/A |
Storage | F/D/L |
Category | GPCR CalciumGPCRAssays |
Related | CellSignaling BiochemicalAssays |
1 Thaw1bottleofCat.#20062atroomtemperaturebeforeuse.
2 Make2.5mMReadiUse™probenecidbuffer:Add1mlofReadiUse™probenecid25mMstABIlizedaqueoussolutionto9mlofHHBS(1XHank’swith20mMHepesbuffer,pH7.0),andmixthemwell.
Note1:10mLof2.5mMReadiuse™probenecidbufferisenoughfor1plate.AliquotandstoretheunusedReadiuse™probenecid25mMstabilizedaqueoussolutionat<-20oC,protectedfromlight.Avoidrepeatedfreeze-thawcycles.
Note2:2.5mMReadiuse™probenecidbuffercontains0.02%Pluronic®F-127,soitisnotnecessarytoaddextraPluronic®F-127inthedye-loadingsolution.
3 Makedye-loadingsolutionforonecellplate:AddDMSOreconstitutedfluorescentcalciumdyes(suchasIndo-1AM,Fura-2AM,Fluo-3AM,Fluo-4AM,Fluo-8AM,Rhod-2AMandRhod-4AM)into2.5mMReadiUse™probenecidbuffer,andmixthemwell.Theworkingsolutionisstableforatleast2hoursatroomtemperature.
References&Citations | CitationExplorer |
TeleocidinA2inhibitshumanproteinase-activatedreceptor2signalingintumorcells
Authors:SonjaStahn,LisaThelen,Ina-MariaAlbrecht,JensBitzer,ThomasHenkel,NicoleELISAbethTeusch
Journal:PharmacologyResearch&Perspectives(2016)
Glutathioneadductsonsarcoplasmic/endoplasmicreticulumCa2+ATPaseCys-674regulateendothelialcellcalciumstoresandangiogenicfunctionaswellaspromoteischemicbloodflowrecovery
Authors:MelissaDThompson,YuMei,RobertMWeisbrod,MarcySilver,PraphullaCShukla,VictoriaMBolotina,RichardACohen,XiaoyongTong
Journal:JournalofBIOLOGicalChemistry(2014):19907--19916