AAT Bioquest/ApoBrite™ V570 caspase 3/7 substrate *Cell-Permeable*/20201/100 Tests-免疫在线蚂蚁淘旗下平台

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商品详细AAT Bioquest/ApoBrite™ V570 caspase 3/7 substrate *Cell-Permeable*/20201/100 Tests

AAT Bioquest/ApoBrite™ V570 caspase 3/7 substrate *Cell-Permeable*/20201/100 Tests

商品编号： 20201

品牌： aatbio

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

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产品分类：其他生物染料

公司分类： Other_biological_dyes

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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Ex/Em (nm)	405/600
MW	N/A
CAS #	N/A
Solvent	DMSO
Storage	F/D/L
Category	Cell Analysis Cell Apoptosis
Related	Apoptosis and Cytotoxicity Biochemical Assays

The activation of caspase 3/7 is important for the initiation of apoptosis. Our Cell Meter™ No-Wash Live Cell Caspase Activity Assay Kits are based on ApoBrite™ V570, our recently developed cell-permeable fluorogenic caspase substrate, the first fluorogenic probe for the direct detection of caspase activities in live cells. ApoBrite V600 consists of three moieties including a). masked fluorophore, b). caspase-selective peptide fragment (DEVD), and c). cell-penetrating moiety. The cell-penetrating moiety carries the probe into live cells. Upon entering live cells the caspase-selective peptide fragment is cleaved by a caspase to release the masked fluorophore. The intensity of recovered fluorescence is directly related to the activity of caspase to be measured. Compared to the existing caspase assays in live cells, ApoBrite™ V570 is much more robust, convenient and accurate. ApoBrite™ V570 releases a fluorophore that has a large Stokes shift with Ex/Em ~405/570, and can be well excited with violet laser that is installed most of new flow cytometers. It does not need a DNA interaction to be fluorescent as reported for NucView reagents. It does not inhibit caspase activity as reported for the FMK peptide probes. Although fluorescent FMK peptide inhibitors of caspases are widely used for detecting caspase activities in live cells, this technology has a few severe limitations: a). FMK caspase inhibitors have high cytotoxicity since FMK peptides bind covalently to active caspases; b). The irreversibly covalent binding of FMK peptides to caspases inhibits caspase activities, causing false positive apoptosis; c). FMK assays have extremely high background, and require intensive washings, resulting in very low through put; d). FMK peptides are not stable in aqueous solutions, and have to be used immediately.

References & Citations

Citation Explorer

Helicobacter pylori Secreted Protein HP1286 Triggers Apoptosis in Macrophages via TNF-Independent and ERK MAPK-Dependent Pathways
Authors: Raquel Tavares, Sushil Kumar Pathak
Journal: Frontiers in Cellular and Infection Microbiology (2017): 58

Death receptor 3 mediates necroptotic cell death
Authors: Sebastian Bittner, Gertrud Knoll, Martin Ehrenschwender
Journal: Cellular and Molecular Life Sciences (2016): 1--12

Helicobacter pylori protein JHP0290 exhibits proliferative and anti-apoptotic effects in gastric epithelial cells
Authors: Raquel Tavares, Sushil Kumar Pathak
Journal: PloS one (2015): e0124407

品牌介绍

美国AAT Bioquest Inc.(前身是ABD Bioquest，Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域，包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品，快速地丰富各个领域的产品。 1）提供反应探针和发光探针，生物素和端粒酶能够应用于标记药物小分子和生物聚合物，如蛋白、核酸以及其他碳水化合物； 2）研究并生产荧光和发光探针用于检测蛋白，核酸和活细胞； 3）不断推出新型的荧光和发光探针用于检测多种酶，特别是检测水解酶和氧化还原酶类； 4）致力于开发用于信号转导研究的试剂； 5）提供生理和神经探针，特别是钙离子指示剂和膜电位探针。

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淋巴细胞信号传导 GPCR抗体羰基活性生物素细胞代谢胺反应生物素链霉亲和素结合物生物素化抗IgGs 乙酰基特异性抗体细胞骨架抗体

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