Overview | PrinterFriendlyVersion |
Ex/Em(nm) | 575/597 |
MW | N/A |
CAS# | N/A |
Solvent | N/A |
Storage | R/L |
Category | CellBIOLOGy pHandIonIndicators |
Related | CellMetabolism BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
1.Preparecellsasdesired.Forexample,preparethegranulocytesat107cells/mLHanksand20mMHepesbuffer(HHBS),add100μLtoapolypropylenetube.
Note: Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensity.
2.Add1-10μLoftheProtonex™Red600-LatexBeadConjugatetothetubeandincubatewithgentleshakingfor30minutesat37˚C.
Note: Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalincubationtime.
3.Prepareanidenticalsamplethatisincubatedat4˚Cascontrol.
4.Attheendofthe30minuteincubation,stopthephagocytosisbyadding2mLoficecoldHHBS.Mixandthenwashthecells2timeswithcoldHBSS.
5.ResUSPendthecellsin500μLofcoldHBSS,keepthesamplesat4˚CandanalyzeassoonaspossIBLeatEx/Em570/600nM(TexasRed®filterset).
References&Citations | CitationExplorer |
PHD2isaregulatorforglycolyticreprogramminginmacrophages
Authors:AnnemarieGuentsch,AngelikaBeneke,LijaSwain,KatjaFarhat,ShunmugamNagarajan,BenWielockx,KaaminiRaithatha,JanDudek,PeterRehling,AnkeZieseniss
Journal:MolecularandCellularBiology(2016):MCB--00236