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AAT Bioquest/CFSE[5-（和6）-羧基荧光素二乙酸酯，琥珀酰亚胺酯]*CAS 150347-59-4*/22022/25 mg
![AAT Bioquest/CFSE[5-（和6）-羧基荧光素二乙酸酯，琥珀酰亚胺酯]*CAS 150347-59-4*/22022/25 mg](https://www.ebiomall.cn/images/AAT201712/chemical-structure-for-cfse-5-and-6-carboxyfluorescein-diacetate-succinimidyl-ester-cas-150347-59-4.png)
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 494/521 |
| MW | 557.46 |
| CAS# | 150347-59-4 |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | CellBIOLOGy LabelingCells |
| Related | ViABIlityandProliferation CellViability BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1.Prepare10mMDMSOstocksolution
For#22022:Dissolve5.6mgin1mlDMSOtomake10mMstocksolution(1mg/mlisequivalentto1.8mM);For#22028add90µLDMSOtoeachvialtomake10mMstocksolution.
Note:Thestocksolutionshouldbeusedpromptly;anyremainingsolutionshouldbealiquotedandfrozenat<-20oC.Avoidrepeatedfreeze-thawcycles,andprotectfromlight.
2.PrepareCFSEworkingsolution
PrepareaCFSEworkingsolution(0.5to5µM)rightbeforeusebydilutingtheDMSOstocksolution(20,000to2,000times)fromStep1withHanksand20mMHepesbuffer(HHBS)orthebufferofyourchoice,pH7.Mixthemwellbyvortexing.
3.Analyzecellswithaflowcytometerorafluorescencemicroscope:
3.1 Treatcellswithtestcompoundsforadesiredperiodoftime.
3.2 Centrifugethecellstoget1-5×105cellspertube.
3.3 ResUSPendcellsin500μLofthedyeworkingsolution(fromStep2).
3.4 Incubatecellswithadyesolutionatroomtemperatureor37°Cfor10to30min,protectedfromlight.
3.5 Removethedyeworkingsolutionfromthecells,washthecellswithHHBSorbufferofyourchoice.Resuspendcellsin500μLofpre-warmedHHBSormediumtoget1-5×105cellspertube.
3.6 MonitorthefluorescencechangeatEx/Em=490/520nmwithaflowcytometer(FL1channel)orafluorescencemicroscope.
| References&Citations |  CitationExplorer |
Fluidandcellbehaviorsalonga3Dprintedalginate/gelatin/fibrinchannel
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OverexpressionoftheCaTIP1-1peppergeneintobaccoenhancesresistancetoosmoticstresses
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IdentificationofVAR2CSAdomain-specificinhibitoryantibodiesofthePlasmodiumfalciparumerythrocytemembraneprotein1usinganovelflowcytometryassay
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Journal:Journalofdermatologicalscience(2011):160--168
