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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Fluorescent_dyes > AAT Bioquest/CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers*/22026/25
商品详细AAT Bioquest/CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers*/22026/25
AAT Bioquest/CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers*/22026/25
AAT Bioquest/CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers*/22026/25
商品编号: 22026
品牌: aatbio
市场价: ¥27560.00
美元价: 16536.00
产地: 美国(厂家直采)
公司:
产品分类: 荧光染料
公司分类: Fluorescent_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
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Ex/Em (nm) 504/529
MW 626.35
CAS # N/A
Solvent DMSO
Storage F/D/L
Category Cell Biology
Labeling Cells
Related Viability and Proliferation
Cell Viability
Biochemical Assays
It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample. Flow cytometry combined with fluorescent staining is a powerful tool to analyze heterogeneous cell populations. Fluorescein diacetate (FDA) and its derivatives are non-fluorescent molecules that diffuse into cells and are hydrolyzed by intracellular non-specific esterases to give fluorescent products. The fluorescent products can be accumulated only in those cells that have intact cell membranes; therefore, dead cells with leaky membranes are not stained. The precise kinetics of membrane transport and intracellular hydrolysis of FDA and its analogs (such as CDCFDA) are related to cellular functions, thus FDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The fluorescence intensity of labeled cells by FDA dyes varies considerably among cell lines and strains, probably because of differences in intracellular esterase activity. 5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester, is an amine-reactive FDA derivative that can be used to prepare a variety of FDA conjugates.
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References & Citations
Citation Explorer

Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel
Authors: Yufan Xu, Xiaohong Wang
Journal: Biotechnology and bioengineering (2015): 1683--1695

Overexpression of the CaTIP1-1 pepper gene in tobacco enhances resistance to osmotic stresses
Authors: Yan-Xu Yin, Shu-Bin Wang, Huai-Juan Xiao, Huai-Xia Zhang, Zhen Zhang, Hua Jing, Ying-Li Zhang, Ru-Gang Chen, Zhen-Hui Gong
Journal: International journal of molecular sciences (2014): 20101--20116

Identification of VAR2CSA domain-specific inhibitory antibodies of the Plasmodium falciparum erythrocyte membrane protein 1 using a novel flow cytometry assay
Authors: Harold Obiakor, Marion Avril, Nicholas J MacDonald, Prakash Srinivasan, Karine Reiter, Charles Anderson, Kevin L Holmes, Michal Fried, Patrick E Duffy, Joseph D Smith
Journal: Clinical and Vaccine Immunology (2013): 433--442

Aging enhances maceration-induced ultrastructural alteration of the epidermis and impairment of skin barrier function
Authors: Takeo Minematsu, Yuko Yamamoto, Takashi Nagase, Ayumi Naito, Kimie Takehara, Shinji Iizaka, Kazunori Komagata, Lijuan Huang, Gojiro Nakagami, Tomoko Akase
Journal: Journal of dermatological science (2011): 160--168


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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