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当前位置: 首页 > 产品中心 > Other_kits > AAT Bioquest/Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Microplate Readers*/
商品详细AAT Bioquest/Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Microplate Readers*/
AAT Bioquest/Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Microplate Readers*/
AAT Bioquest/Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Microplate Readers*/
商品编号: 22791
品牌: aatbio
市场价: ¥56560.00
美元价: 33936.00
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)498/520
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryCellAnalysis
CellCytotoxicity
RelatedApoptosisandCytotoxicity
CellApoptosis
BiochemicalAssays
OurCellMeter™assaykitsareasetoftoolsformonitoringcellviABIlity.Thereareavarietyofparametersthatcanbeusedformonitoringcellviability.Thisparticularkitisdesignedtomonitorcellapoptosisthroughmeasuringthetranslocationofphosphatidylserine(PS).Inapoptosis,PSistransferredtotheouterleafletoftheplasmamembrane.Theappearanceofphosphatidylserineonthecellsurfaceisauniversalindicatoroftheinitial/intermediatestagesofcellapoptosisandcanbedetectedbeforemorphologicalchangescanbeobserved.ThiskitusesafluorescentsensorthatspecificallybindsPS.Thiskitusesourproprietaryfluorescentsmallmolecule-basedApopxin™PSsensorthatspecificallybindsPSwithaffinitymuchhigherthanAnnexinV(Kd<10=""nm).=""it=""has=""green=""fluorescence=""upon=""binding=""to=""membrane=""ps.=""it=""can=""be=""used=""in=""the=""formats=""of=""microplate,=""microscope=""and=""flow=""cytometer=""while=""most=""of=""other=""commercial=""apoptosis=""assay=""kits=""are=""only=""used=""with=""either=""microscope=""or=""flow=""cytometry=""platform.="">
SpectrumAdvancedSpectrumViewer



Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Preparecells:

1.1   Foradherentcells:Platecellsovernightingrowthmediumat20,000cells/well/90µLfora96-wellplateor5,000cells/well/20µLfora384-wellplate.

1.2   Fornon-adherentcells:CentrifugethecellsfromtheculturemediumandthensUSPendthecellpelletinculturemediumat80,000to200,000cells/well/90µLfora96-wellpoly-Dlysineplateor20,000to50,000cells/well/20µLfora384-wellpoly-Dlysineplate.Centrifugetheplateat800rpmfor2minuteswithbrakeoffpriortotheexperiments.

Note:Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensityforapoptosisinduction.

 

2.PrepareApopxin™Greenassayworkingsolution:

2.1   WarmKitComponentBatroomtemperaturebeforeuse.

2.2   Add10µLofApopxin™Green(ComponentA)into1mLofAssayBuffer(ComponentB),andmixthemwell.

Note:100µLofApopxin™Greenassayworkingsolutionisenoughforonewell.Preparefreshbeforeuse.

 
3.Runapoptosisassay:

3.1   Treatcellswithtestcompoundsbyadding10µL/well(96-wellplate)or2.5µL/well(384-wellplate)of10XtestcompoundstocksolutionintoPBSorthedesiredbuffer.Forblankwells(mediumwithoutthecells),addthesameamountofcompoundbuffer.

3.2   Incubatethecellplateina5%CO2,37°Cincubatorforadesiredperiodoftime(4-6hoursforJurkatcellstreatedwithcamptothecin)toinduceapoptosis.

3.3    Add100µL/well(96-wellplate)or25µL/well(384-wellplate)of2XApopxin™Greenassayworkingsolution(fromStep2.2)intoeachwell.

3.4    Incubatethecellplateatroomtemperatureforatleast1hour,protectedfromlight.

3.5    Centrifugecellplate(especiallyforthenon-adherentcells)at800rpmfor2minutes(brakeoff).

3.6    MonitorthefluorescenceintensityatEx/Em=490/525nm(cutoffat510nm)byusingafluorescentmicroplatereader(bottomreadmode)orusingafluorescentmicroscope(FITCchannel).

References&Citations
CitationExplorer

PhysiologicaleffectsoftheherbicideglyphosateonthecyanobacteriumMicrocystisaeruginosa
Authors:LiangWu,ZhihaoQiu,YaZhou,YupingDu,ChaonanLiu,JingYe,XiaojunHu
Journal:AquaticToxicology(2016):72--79

Tumor-selectivemitochondrialnetworkcollapseinducedbyatmosphericgasplasma-activatedmedium.
Authors:KosukeSaito,TomohikoAsai,KyokoFujiwara,JunkiSahara,HaruhisaKoguchi,NoboruFukuda,MikiSuzuki-Karasaki,MasayoshiSoma,YoshihiroSuzuki-Karasaki
Journal:Oncotarget(2016)

Inhibitionofmalignantphenotypesofhumanosteosarcomacellsbyagenesilencer,apyrrole--imidazolepolyamide,whichtargetsanE-boxmotif
Authors:MasashiTaniguchi,KyokoFujiwara,YujiNakai,ToshinoriOzaki,NobukoKoshikawa,KojimaToshio,MotoakiKataba,AsakoOguni,HiroyukiMatsuda,YukihiroYoshida
Journal:FEBSopenbio(2014):328--334


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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