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AAT Bioquest/Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry*/22810/100 Tests
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 490/515 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | CellAnalysis CellCytotoxicity |
| Related | ApoptosisandCytotoxicity CellApoptosis BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
Note:Thawallthekitcomponentsatroomtemperaturebeforeuse.
1.Preparecells:
Foreachsample,preparecellsin1mLwarmmediumorbufferofyourchoiceatadensityof5×105to1×106cells/mL.
Note:Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensityforapoptosisinduction.
2.Prepare200XThiolite™Green:
Add500µLofDMSO(ComponentC)intothevialofThiolite™Green(ComponentA),andmixwell.
Note:AliquotandstoredtheunusedComponentAat-20oC.Avoidrepeatedfreeze/thawcycles.
3.RunGSHGreen™Assay:
3.1 Treatcellswithtestcompoundsforadesiredperiodoftimetoinduceapoptosis.
3.2 Add5μLof200XThiolite™Green(fromStep2),andincubatethecellsina37°C,5%CO2incubatorfor15to30minutes.
Note1:Foradherentcells,gentlyliftthecellswith0.5mMEDTAtokeepthecellsintact,andwashthecellsoncewithserum-containingmediapriortotheincubationwithThiolite™Greendye-loadingsolution.
Note2:Theappropriateincubationtimedependsontheindividualcelltypeandcellconcentrationused.Optimizetheincubationtimeforeachexperiment.
3.3 Optional:Centrifugethecellsat1000rpmfor4minutes,andthenre-sUSPendcellsin1mLofAssayBuffer(ComponentB)orbufferofyourchoice.
3.4 MonitorthefluorescenceintensitywithaflowcytometerusingtheFL1channel(Ex/Em=490/525nm).Gateonthecellsofinterest,excludingdebris.
| References&Citations |  CitationExplorer |
ROSproductionandglutathioneresponseinkeratinocytesafterapplicationofβ-caroteneandVIS/NIRirradiation
Authors:SilkeBLohan,KristinaVitt,PatrikScholz,CorneliaMKeck,MartinaCMeinke
Journal:Chemico-BIOLOGicalinteractions(2017)
OsmoticstressisaccompaniedbyproteinglycationinArabidopsisthaliana
Authors:GaganPaudel,TatianaBilova,RicoSchmidt,UtaGreifenhagen,RobertBerger,ElenaTarakhovskaya,StefanieStöckhardt,GerdUlrichBalcke,KlausHumbeck,WolfgangBrandt
Journal:JournalofExperimentalBotany(2016):6283--6295
SystemicInductionofNO-,Redox-,andcGMPSignalinginthePumpkinExtrafascicularPhloemuponLocalLeafWounding
Authors:FrankGaupels,AlexandraCUFurch,MatthiasRZimmermann,FaxingChen,VolkhardKaever,AnjaBuhtz,JuliaKehr,HakanSarioglu,Karl-HeinzKogel,JörgDurner
Journal:Frontiersinplantscience(2016)
Predictionofintracellularmetabolicstatesfromextracellularmetabolomicdata
Authors:MaikeKAurich,GiuseppePaglia,OttarRolfsson,SigrúnHrafnsdóttir,ManuelaMagnúsdóttir,MagdalenaMStefaniak,BernhardOPalsson,RonanMTFleming,InesThiele
Journal:Metabolomics(2015):603--619
Molecularmechanismsofhyperthermia-inducedapoptosisenhancedbywithaferinA
Authors:Zheng-GuoCui,Jin-LanPiao,MatiURRehman,RyoheiOgawa,PengLi,Qing-LiZhao,TakashiKondo,HidekuniInadera
Journal:Europeanjournalofpharmacology(2014):99--107
Cancer&Metabolism
Authors:GregoryLaMonte,XiaohuTang,JuliaLing-YuChen,JianliWu,Chien-KuangCorneliaDing,MelissaMKeenan,CarolynSangokoya,Hsiu-NiKung,OlgaIlkayeva,LászlóGBoros
Journal:(2013)
