| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 503/526 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | CellAnalysis CellCytotoxicity |
| Related | ApoptosisandCytotoxicity CellApoptosis BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1.Foreachsample,preparecellsin1mLofwarmmediumorbufferofyourchoiceatadensityof5×105to1×106cells/mL.
Note:Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensityforapoptosisinduction.
2.Treatcellswithtestcompoundsforadesiredperiodoftimetoinduceapoptosis.
3.Add5μLof200XNuclearGreen™DCS1(ComponentA)intothetreatedcells(fromStep2),andincubatethecellsolutionina37°C,5%CO2incubatorfor30to60minutes.
Note1:Foradherentcells,gentlyliftthecellswith0.5mMEDTAtokeepthecellsintack,andwashthecellsoncewithserum-containingmediapriortotheincubationwithNuclearGreen™DCS1dye-loADIngsolution.
Note2:Theappropriateincubationtimedependsontheindividualcelltypeandcellconcentrationused.Optimizetheincubationtimeforeachexperiment.
4.Optional:Centrifugethecellsat1000rpmfor4minutes,andthenre-sUSPendcellsin1mLofAssayBuffer(ComponentB)orbufferofyourchoice.
5.MonitorthefluorescenceintensitywithaflowcytometerusingtheFL1channel(Ex/Em=490/525nm).Gateonthecellsofinterest,excludingdebris.
| References&Citations |  PrinterFriendlyVersion |
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