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AAT Bioquest/Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*/22824
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 490/520 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | CellAnalysis CellCytotoxicity |
| Related | ApoptosisandCytotoxicity CellApoptosis BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1. Treatcellswithtestcompoundsforadesiredperiodoftime(4-6hoursforJurkatcellstreatedwithcamptothecin)toinduceapoptosis.
Note:AnnexinVflowcytometricanalysisonadherentcellsisnotroutinelytestedsincespecificmembranedamagemayoccurduringcelldetachmentorharvesting.However,methodsforutilizingAnnexinVforflowcytometryonadherentcelltypeshavebeenpreviouslyreportedbyCasiola-Rosenetal.andvanEngelendetal(seeRefs1and2).
2. Centrifugethecellstoget1-5×105cells/tube.
3. ResUSPendcellsin200μLofAssayBuffer(ComponentB).
4. Add2μLofAnnexinV-iFluor™488(ComponentA)intothecells.
Optional:Add2µLof100XPropidiumIodide(ComponentC)fornecrosiscells.
5. Incubateatroomtemperaturefor30to60minutes,protectedfromlight.
6. Optional:add200to300μLofAssayBuffer(ComponentB)toincreasevolumebeforeanalyzingthecellswithaflowcytometer(SeeStep7).
7. MonitorthefluorescenceintensityofAnnexinV-iFluor™488usingtheFL1channel(Ex/Em=490/525nm),andmeasurethecellviabilitywithpropidiumiodideusingtheFL2channel.
| References&Citations |  CitationExplorer |
Insitupolymerizablehydrogelincorporatedwithspecificpathogen-freeporcineplatelet-richplasmaforthereconstructionofthecornealendothelium
Authors:Yung-KaiLin,RuchiSharma,HsuMa,Wen-ShyanChen,Chao-LingYao
Journal:JournaloftheTaiwanInstituteofChemicalEngineers(2017)
NovelregulationsofMEF2-A,MEF2-D,andCACNA1SinthefunctionalincompetenceofADIpose-derivedmesenchymalstemcellsbyinducedindoxylsulfateinchronickidneydisease
Authors:DuyenThiDo,NamNhutPhan,Chih-YangWang,ZhengdaSun,Yen-ChangLin
Journal:Cytotechnology(2016):2589--2604
Shearstress-inducedalterationofepithelialorganizationinhumanrenaltubularcells
Authors:DamienMaggiorani,RomainDissard,MarcyBelloy,Jean-SébastienSaulnier-Blache,AudreyCasemayou,LaureDucasse,SandraGrès,JulieBellière,CécileCaubet,Jean-LoupBascands
Journal:PloSone(2015):e0131416
TargetingaG-protein-coupledreceptoroverexpressedinendocrinetumorsbymagneticnanoparticlestoinducecelldeath
Authors:ClaireSanchez,DarineElHajjDiab,VincentConnord,PascalClerc,EtienneMeunier,BernardPipy,BrunoPayré,ReasmeyPTan,MichelGougeon,JulianCarrey
Journal:AcsNano(2014):1350--1363
