Overview | PrinterFriendlyVersion |
Ex/Em(nm) | 520/547 |
MW | ~1800 |
CAS# | N/A |
Solvent | DMSO |
Storage | F/D/L |
Category | CellBIOLOGy LabelingCells |
Related | BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
1.Prepare1000XPhalloidinDMSOstocksolution:byadding30uLofDMSOintothepowderformvials.
2.Prepare1XPhalloidinconjugateworkingsolution:byadding1uLof1000XPhalloidinconjugateDMSOsolutionto
1mLofPBSwith1%BSA.
Note1:Theunused1000XDMSOstocksolutionofphalloidinconjugateshouldbealiquotedandstoredat-20oC.protectedfromlight.
Note2:Differentcelltypesmightbestaineddifferently.Theconcentrationofphalloidinconjugateworkingsolutionshouldbepreparedaccordingly.
3.Stainthecells:
3.1 Performformaldehydefixation.Incubatecellswith3.0–4.0%formaldehydeinPBSatroomtemperaturefor10–30minutes.
Note:Avoidanymethanolcontainingfixativessincemethanolcandisruptactinduringthefixationprocess.Thepreferredfixativeismethanol-freeformaldehyde.
3.2 Rinsethefixedcells2–3timesinPBS.
3.3 Optional:Add0.1%TritonX-100inPBSintofixedcells(fromStep2.2)for3to5minutestoincreasepermeability.Rinsethecells2–3timesinPBS.
3.4 Add100uL/well(96-wellplate)ofphalloidinconjugateworkingsolution(fromStep1)intothefixedcells(fromStep2.2or2.3),andstainthecellsatroomtemperaturefor20to90minutes.
3.5 RinsecellsgentlywithPBS2to3timestoremoveexcessphalloidinconjugatebeforeplating,sealingandimagingundermicroscope.
References&Citations | CitationExplorer |
BiomaterialSurfaceCanModifyHUVECMorphologyandInflammatoryResponsebyRegulatingMicroRNAExpression
Authors:ShuangyingGu,BaoxiangTian,WeicongChen,YueZhou
Journal:JournalofBiosciencesandMedicines(2017):8
Cell-Permeable,MMP-2Activatable,NickelFerriteandHis-taggedFusionProteinSelf-AssembledFluorescentNanoprobeforTumorMagneticTargetingandImaging
Authors:LuSun,ShupingXie,JingQi,ErgangLiu,DiLiu,QuanLiu,SunhuiChen,HuiningHe,VictorCYang
Journal:ACSAppliedMaterials&Interfaces(2017)
DNADouble-StrandBreaksInducetheNuclearActinFilamentsFormationinCumulus-EnclosedOocytesbutNotinDenudedOocytes
Authors:Ming-HongSun,MoYang,Feng-YunXie,WeiWang,LiliZhang,WeiShen,ShenYin,Jun-YuMa
Journal:PloSone(2017):e0170308
DNADouble-StrandBreaksInducetheNuclearActinFilamentsFormationinCumulus-EnclosedOocytesbutNotinDenudedOocytes
Authors:Ming-HongSun,MoYang,Feng-YunXie,WeiWang,LiliZhang,WeiShen,ShenYin,Jun-YuMa
Journal:PloSone(2017):e0170308
EnhancedbovineserumalbuminabsorptionontheN-hydroxysuccinimideactivatedgrapheneoxideanditscorrespondingcellaffinity
Authors:KunXiong,QingboFan,TingtingWu,HaishanShi,LinChen,MinhaoYan
Journal:MaterialsScienceandEngineering:C(2017)
Enhancedosteointegrationoftantalum-modifiedtitaniumimplantswithmicro/nano-topography
Authors:JunyuShi,XiaomengZhang,ShichongQiao,JieNi,JiajiMo,YingxinGu,HongchangLai
Journal:RSCAdvances(2017):46472--46479
GraftingofRing-OpenedCyclopropylaminethinfilmsonSilicon(100)HydrideviaUVPhotoionization
Authors:JolineTung,JingYuanChing,YokeMooiNg,LihShinTew,YitLungKhung
Journal:ACSAppliedMaterials&Interfaces(2017)
MicrotopographyAttenuatesEndothelialCellProliferationbyRegulatingMicroRNAs
Authors:DanWang,MengyaLiu,ShuangyingGu,YueZhou,SongLi
Journal:JournalofBiomaterialsandNanobiotechnology(2017):189--201
StudyontheRegulationofFocalAdesionsandCorticalActinbyMatrixNanotopographyin3DEnvironment
Authors:JingjingHan,Keng-huiLin,LockYueChew
Journal:JournalofPhysics:CondensedMatter(2017)
Thecorrelationbetweenosteopontinadsorptionandcelladhesiontomixedself-assembledmonolayersofvaryingchargesandwettability
Authors:LijingHao,TianjieLi,FanYang,NaruZhao,FuzhaiCui,XuetaoShi,ChangDu,YingjunWang
Journal:BiomaterialsScience(2017)