| Overview |
 Printer Friendly Version
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| Ex/Em (nm) | 752/778 |
| MW | ~3300 |
| CAS # | N/A |
| Solvent | DMSO |
| Storage | F/D/L |
| Category |
Cell Biology Labeling Cells |
| Related |
Biochemical Assays |
| Spectrum | Advanced Spectrum Viewer |
1. Prepare 1000 X Phalloidin DMSO stock solution: by adding 30 uL of DMSO into the powder form vials.
2. Prepare 1X Phalloidin conjugate working solution: by adding 1 uL of 1000X Phalloidin conjugate DMSO solution to
1 mL of PBS with 1% BSA.
Note 1: The unused 1000X DMSO stock solution of phalloidin conjugate should be aliquoted and stored at -20 oC. protected from light.
Note 2: Different cell types might be stained differently. The concentration of phalloidin conjugate working solution should be prepared accordingly.
3. Stain the cells:
3.1 Perform formaldehyde fixation. Incubate cells with 3.0–4.0 % formaldehyde in PBS at room temperature for 10–30 minutes.
Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.
3.2 Rinse the fixed cells 2–3 times in PBS.
3.3 Optional: Add 0.1% Triton X-100 in PBS into fixed cells (from Step 2.2) for 3 to 5 minutes to increase permeability. Rinse the cells 2–3 times in PBS.
3.4 Add 100 uL/well (96-well plate) of phalloidin conjugate working solution (from Step 1) into the fixed cells (from Step 2.2 or 2.3), and stain the cells at room temperature for 20 to 90 minutes.
3.5 Rinse cells gently with PBS 2 to 3 times to remove excess phalloidin conjugate before plating, sealing and imaging under microscope.
| References & Citations |
 Citation Explorer
|
Biomaterial Surface Can Modify HUVEC Morphology and Inflammatory Response by Regulating MicroRNA Expression
Authors: Shuangying Gu, Baoxiang Tian, Weicong Chen, Yue Zhou
Journal: Journal of Biosciences and Medicines (2017): 8
Cell-Permeable, MMP-2 Activatable, Nickel Ferrite and His-tagged Fusion Protein Self-Assembled Fluorescent Nanoprobe for Tumor Magnetic Targeting and Imaging
Authors: Lu Sun, Shuping Xie, Jing Qi, Ergang Liu, Di Liu, Quan Liu, Sunhui Chen, Huining He, Victor C Yang
Journal: ACS Applied Materials & Interfaces (2017)
DNA Double-Strand Breaks Induce the Nuclear Actin Filaments Formation in Cumulus-Enclosed Oocytes but Not in Denuded Oocytes
Authors: Ming-Hong Sun, Mo Yang, Feng-Yun Xie, Wei Wang, Lili Zhang, Wei Shen, Shen Yin, Jun-Yu Ma
Journal: PloS one (2017): e0170308
DNA Double-Strand Breaks Induce the Nuclear Actin Filaments Formation in Cumulus-Enclosed Oocytes but Not in Denuded Oocytes
Authors: Ming-Hong Sun, Mo Yang, Feng-Yun Xie, Wei Wang, Lili Zhang, Wei Shen, Shen Yin, Jun-Yu Ma
Journal: PloS one (2017): e0170308
Enhanced bovine serum albumin absorption on the N-hydroxysuccinimide activated graphene oxide and its corresponding cell affinity
Authors: Kun Xiong, Qingbo Fan, Tingting Wu, Haishan Shi, Lin Chen, Minhao Yan
Journal: Materials Science and Engineering: C (2017)
Enhanced osteointegration of tantalum-modified titanium implants with micro/nano-topography
Authors: Junyu Shi, Xiaomeng Zhang, Shichong Qiao, Jie Ni, Jiaji Mo, Yingxin Gu, Hongchang Lai
Journal: RSC Advances (2017): 46472--46479
Grafting of Ring-Opened Cyclopropylamine thin films on Silicon (100) Hydride via UV Photoionization
Authors: Joline Tung, Jing Yuan Ching, Yoke Mooi Ng, Lih Shin Tew, Yit Lung Khung
Journal: ACS Applied Materials & Interfaces (2017)
Microtopography Attenuates Endothelial Cell Proliferation by Regulating MicroRNAs
Authors: Dan Wang, Mengya Liu, Shuangying Gu, Yue Zhou, Song Li
Journal: Journal of Biomaterials and Nanobiotechnology (2017): 189--201
Study on the Regulation of Focal Adesions and Cortical Actin by Matrix Nanotopography in 3D Environment
Authors: Jingjing Han, Keng-hui Lin, Lock Yue Chew
Journal: Journal of Physics: Condensed Matter (2017)
The correlation between osteopontin adsorption and cell adhesion to mixed self-assembled monolayers of varying charges and wettability
Authors: Lijing Hao, Tianjie Li, Fan Yang, Naru Zhao, Fuzhai Cui, Xuetao Shi, Chang Du, Yingjun Wang
Journal: Biomaterials Science (2017)
