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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT Bioquest/Cell Meter™ Colorimetric WST-8 Cell Quantification Kit/22770/1000 Tests
商品详细AAT Bioquest/Cell Meter™ Colorimetric WST-8 Cell Quantification Kit/22770/1000 Tests
AAT Bioquest/Cell Meter™ Colorimetric WST-8 Cell Quantification Kit/22770/1000 Tests
AAT Bioquest/Cell Meter™ Colorimetric WST-8 Cell Quantification Kit/22770/1000 Tests
商品编号: 22770
品牌: aatbio
市场价: ¥56560.00
美元价: 33936.00
产地: 美国(厂家直采)
公司:
产品分类: 其他生物染料
公司分类: Other_biological_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)460/None
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryCellAnalysis
CellCytotoxicity
RelatedApoptosisandCytotoxicity
CellApoptosis
OurCellMeter™assaykitsareasetoftoolsformonitoringcellviABIlity.Thereareavarietyofparametersthatcanbeusedformonitoringcellviability.CellMeter™ColorimetricWST-8CellQuantificationKituseswater-solubleWST-8tetrazoliumsalttoquantifythenumberoflivecells.Thewater-solubleWST-8tetrazoliumsaltproducesawater-solubleorangeformazandyeuponbioreductioninthepresenceofanelectroncarrier,1-methoxy-5-methylphenaziniummethylsulfate.Thekitisconvenientandrobustwithamixandreadformat.WST-8solutionisaddeddirectlytothetestcells,nopre-mixingofcomponentsisrequired.WST-8tetrazoliumsaltisreducedbycellulardehydrogenasestoanorangeformazanproductthatissolubleintissueculturemedium.Theamountofformazanproducedisdirectlyproportionaltothenumberoflivingcellsbymonitoringabsorbanceincreaseat460nm.TheexcellentstabilityandlittlecytotoxicityofWST-8solutionmakethekitusefulfortheassaysthatrequirelongincubation(suchas24to48hours).CellMeter™ColorimetricWST-8CellQuantificationKitprovidesasensitivecolorimetricassayforthedeterminationofthenumberofviablecellsintheproliferationandcytotoxicityassays.Thedetectionsensitivityishigherthananyothertetrazoliumsalt-basedassayssuchasMTT,XTTorMTSetc.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. CellProliferationandCytotoxicityAssay
    1. Plate5000to10,000cells/wellinatissueculturemicroplatewithclearbottom.Addtestcompoundsintothecellsandincubateforadesiredperiodoftime(suchas24,48or96hours)ina37ºC,5%CO2incubator.Forblankwells(mediumwithoutthecells),addthesameamountoftestcompounds.Thesuggestedtotalvolumeis100μLfora96-wellplate,and50μLfora384-wellplate.
      Note:Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensityforproliferationorcytotoxicityinduction.Forproliferationassays,usefewercells;forcytotoxicityassays,usemorecellstostartwith.
    2. Add10μL/well(96-wellplate)or5μL/well(384-wellplate)ofWST-8Solutiontoeachwell.Incubatetheplateat37ºCfor1-4hours,andprotectfromlight.
      Note:Theincubationtimecouldbefrom30minutestoovernightdependingontheindividualcelltypeandcellconcentrationused.Optimizetheincubationtimeforeachexperiment.
    3. MonitortheabsorbanceincreasewithanabsorbanceplatereaderatOD=460nm.
  2. CellCountingAssay
    1. Preparecellcultureinatissueculturemicroplatewithclearbottom.Thesuggestedtotalvolumeis100μLfora96-wellplate,and50μLfora384-wellplate.
      Note:WeusedseriallydilutedHeLaandJurkatcellsUSPensioninaclearbottom96-wellplatefortheassay.SeeFigure2fordetails.
    2. Add10μL/well(96-wellplate)or5μL/well(384-wellplate)ofWST-8Solutiontoeachwell.Incubatetheplateat37ºCfor1-4hours,andprotectfromlight.
      Note:Theincubationtimecouldbefrom30minutestoovernightdependingontheindividualcelltypeandcellconcentrationused.Optimizetheincubationtimeforeachexperiment.
    3. MonitortheabsorbanceincreasewithanabsorbanceplatereaderatOD=460nm
References&Citations
CitationExplorer

miR-218inhibitstheproliferationofhumangliomacellsthroughdownregulationofYinYang1
Authors:YongGao,LaishengSun,ZichengWu,ChengminXuan,JunxiaZhang,YongpingYou,XinchengChen
Journal:MolecularMedicineReports(2017)


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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