AAT Bioquest/Cell Meter™ Multiplexing Live, Apoptotic and Necrotic Cell Detection Kit III *Triple Fluorescence Colors*-免疫在线蚂蚁淘旗下平台

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商品详细AAT Bioquest/Cell Meter™ Multiplexing Live, Apoptotic and Necrotic Cell Detection Kit III *Triple Fluorescence Colors*

AAT Bioquest/Cell Meter™ Multiplexing Live, Apoptotic and Necrotic Cell Detection Kit III *Triple Fluorescence Colors*

商品编号： 22846

品牌： aatbio

市场价： ¥143560.00

美元价： 86136.00

产地：美国(厂家直采)

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产品分类：荧光染料

公司分类： Fluorescent_dyes

联系Q Q： 3392242852

电话号码： 4000-520-616

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Ex/Em(nm)	None/None
MW	N/A
CAS#	N/A
Solvent	N/A
Storage	F/D/L
Category	CellAnalysis CellCytotoxicity
Related	ApoptosisandCytotoxicity CellApoptosis

OurCellMeter™assaykitsareasetoftoolsformonitoringcellviABIlity.Thereareavarietyofparametersthatcanbeused.Thisparticularkitisdesignedtosimultaneouslymonitorapoptotic,necroticandhealthycells.Apoptosisisanactive,programmedprocessofautonomouscellulardismantlingthatavoidselicitinginflammation.Inapoptosis,phosphatidylserine(PS)istransferredtotheouterleafletoftheplasmamembrane.Asauniversalindicatoroftheinitial/intermediatestagesofcellapoptosis,theappearanceofphosphatidylserineonthecellsurfacecanbedetectedbeforemorphologicalchangesareobserved.ThePSsensorAnnexinV-iFluor™647conjugatehasredfluorescence(Ex/Em=620/660nm)uponbindingtomembranePS.Necrosishasbeencharacterizedaspassive,accidentalcelldeathresultingfromenvironmentalperturbationswithuncontrolledreleaseofinflammatorycellularcontents.Lossofplasmamembraneintegrity,asdemonstratedbytheabilityofamembrane-impermeableNuclearBlue™DCS1(Ex/Em=360/450nm)tolabelthenucleus,representsastraightforwardapproachtodemonstratelatestageofapoptosisandnecrosis.Inaddition,thiskitalsoprovidesalivecelllabelingdye,Cellbrite™Orange(Ex/Em=550/570nm),forlabelingnon-apoptotichealthycells.Thiskitisoptimizedtosimultaneouslydetectcellapoptosis(red),necrosis(blueand/orred)andhealthycells(orange)withafluorescencemicroscope.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

Preparecells:
Plate100to100,000cells/wellinatissueculturemicroplatewithblackwallandclearbottom.Addtestcompoundsintothecellsandincubateforadesiredperiodoftime(suchas24,48or96hours)ina37°C,5%CO2incubator.Forblankwells(mediumwithoutthecells),addthesameamountofcompoundbuffer.Thesuggestedtotalvolumeis100µL/well/96-wellplateand25µL/well/384-wellplate.
Note:WetreatedHeLacellswithstaurosporine(SS)for4hoursat37ºCtoinducecellapoptosis.SeeFigure1fordetails.

Preparetriplefluorescenceassaysolution:
1. Preparetriplefluorescenceassaysolutionbyadding10μLof100XAnnexinV-iFluor™488conjugate(ComponentA),5μLof200XNuclearBlue™DCS1(ComponentC)and5μLof200XCellbrite™Red(ComponentD)to1mLofAssayBuffer(ComponentB).Thetriplefluorescenceassaysolutionisstableforatleast1houratroomtemperature.
  Note:Astheoptimalstainingconditionsmayvarydependingondifferentcelltypes,it’srecommendedtodeterminetheappropriateconcentrationofComponentA,CandDindividually.
2. Removecellculturemediumandtestcompoundsaftertreatment(fromStep1).
3. Add100µL/well(96-wellplate)or25µL/well(384-wellplate)oftriplefluorescenceassaysolution.Incubateatroomtemperatureor37ºCfor30to60minutes,protectedfromlight.
4. WashcellswithHBSS,PBSorbufferofyourchoicetwice.
5. AnalyzetheapoptoticcellswithAnnexinV-iFluor™488underfluorescencemicroscopewithaFITCfilter.ThegreenstainingontheplasmamembraneindicatestheAnnexinV-iFluor™488conjugatebindingtoPSoncellsurface.MonitorthefluorescenceintensitywithaDAPIfilterfornecrosis,TexasRedorCy5filterforlivecellsusingafluorescencemicroscope(SeeFigure1fordetails).

References&Citations

CitationExplorer

Anthocyanin-richblackcurrantextractinhibitsproliferationoftheMCF10AhealthyhumanbreastepithelialcelllinethroughinductionofG0/G1arrestandapoptosis
Authors:NaokiNanashima,KayoHorie,MitsuruChiba,ManabuNakano,HayatoMaeda,ToshiyaNakamura
Journal:MolecularMedicineReports(2017):6134--6141

ClusterinsignalsviaApoER2/VLDLRandinducesmeiosisofmalegermcells
Authors:MuhammadAssadRiaz,AngelikaStammler,MareikeBorgers,LutzKonrad
Journal:AmericanJournalofTranslationalResearch(2017):1266

DetectingApoptosis,Autophagy,andNecrosis
Authors:JackColeman,RuiLiu,KathyWang,ArunKumar
Journal:ApoptosisMethodsinToxicology(2016):77--92

品牌介绍

美国AAT Bioquest Inc.(前身是ABD Bioquest，Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域，包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品，快速地丰富各个领域的产品。 1）提供反应探针和发光探针，生物素和端粒酶能够应用于标记药物小分子和生物聚合物，如蛋白、核酸以及其他碳水化合物； 2）研究并生产荧光和发光探针用于检测蛋白，核酸和活细胞； 3）不断推出新型的荧光和发光探针用于检测多种酶，特别是检测水解酶和氧化还原酶类； 4）致力于开发用于信号转导研究的试剂； 5）提供生理和神经探针，特别是钙离子指示剂和膜电位探针。

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淋巴细胞信号传导 GPCR抗体羰基活性生物素细胞代谢胺反应生物素链霉亲和素结合物生物素化抗IgGs 乙酰基特异性抗体细胞骨架抗体

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