| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 360/450 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | SmallMoleculeDetection DiagnosticMolecules |
| Related | RedoxEnzymes BiochemicalAssays |
1.Prepare250XAldeLight™Bluestocksolution:
Add40µLofDMSO(ComponentE)intothevialofAldeLight™Blue(ComponentA)tomake250XAldeLight™Bluestocksolution.
Note:TheunusedAldeLight™Bluestocksolutionshouldbedividedintosingleusealiquots,andstoredat-20oC.
2.PrepareAldeLight™Bluereactionmixture:
Add20µLof250XAldeLight™Bluestocksolution(fromStep1)into5mLofAssayBuffer(ComponentB),andmixthemwell.
Note:5mLofAldeLight™Bluereactionmixtureisenoughforoneplate.Thereactionmixtureisnotstable,andbestusedwithin2hours.
3.Prepareserialdilutionsofaldehydestandard(0to1mM):
3.1 Add1mLofAssayBuffer(ComponentB)intothevialofAldehydeStandard(ComponentD)tomakea10mMaldehydestandardstocksolution.
Note:Theunused10mMAldehydestandardstocksolutionshouldbedividedintosingleusealiquotsandstoredat-20oC.
3.2 Take100μLof10mMaldehydestandardstocksolution(fromStep3.1)toperform1:10,and1:3serialdilutionstoget1000,300,100,30,10,3,1and0μMserialdilutionsofaldehydestandard.
3.3 Addseriallydilutedaldehydestandardsandaldehyde-containingtestsamplesintoasolidblack96-wellmicroplateasdescribedinTables1and2.
Table1.LayoutofAldehydeStandardsandtestsamplesinasolidblack96-wellmicroplate
BL | BL | TS | TS | …. | …. |
|
|
|
|
|
|
AS1 | AS1 | …. | …. | …. | …. |
|
|
|
|
|
|
AS2 | AS2 |
|
|
|
|
|
|
|
|
|
|
AS3 | AS3 |
|
|
|
|
|
|
|
|
|
|
AS4 | AS4 |
|
|
|
|
|
|
|
|
|
|
AS5 | AS5 |
|
|
|
|
|
|
|
|
|
|
AS6 | AS6 |
|
|
|
|
|
|
|
|
|
|
AS7 | AS7 |
|
|
|
|
|
|
|
|
|
|
Note:AS=AldehydeStandards,BL=BlankControl,TS=TestSamples.
Table2.Reagentcompositionforeachwell
AldehydeStandard | BlankControl | TestSample |
SerialDilutions*:50μL | AssayBuffer:50μL | 50μL |
*Note:AddtheseriallydilutedAldehydestandardsfrom1μMto1000μM intowellsfromAS1toAS7induplicate.
4.Runaldehydeassay:
4.1 Add50μLofAldeLight™Bluereactionmixture(fromStep2)intoeachwellofaldehydestandard,blankcontrol,andtestsamples(seeStep3.3)tomakethetotalaldehydeassayvolumeof100µL/well.
Note:Fora384-wellplate,add25μLoftestsampleand25μLofAldeLight™Bluereactionmixtureintoeachwell.
4.2 Incubatethereactionmixtureatroomtemperaturefor15to30minutes,protectedfromlight.
4.3 Add25μLofReactionBuffer(ComponentC)intoeachwell(fromStep4.2).
4.4 MonitorthefluorescenceincreaseatEx/Em=365/435nmusingafluorescenceplatereader.
| References&Citations |  CitationExplorer |
BiologicalActivityofPeptide-conjugatedPolyionComplexMatricesConsistingofAlginateandChitosan
Authors:ChikaraFujimori,JunKumai,KyotaroNakamura,YingziGu,FumihikoKatagiri,KentaroHozumi,YamatoKikkawa,MotoyoshiNomizu
Journal:PeptideScience(2016)
HepaticDeficiencyofAugmenterofLiverRegenerationExacerbatesAlcohol-InducedLiverInjuryandPromotesFibrosisinMice
Authors:SudhirKumar,JiangWang,RichaRani,ChandrashekharRGandhi
Journal:PloSone(2016):e0147864
Integratedself-assemblingdrugdeliverysystempossessingdualresponsiveandactivetargetingfororthotopicovariancancertheranostics
Authors:Chun-JuiLin,Chen-HsiangKuan,Li-WenWang,Hsi-ChinWu,YunchingChen,Chien-WenChang,Rih-YangHuang,Tzu-WeiWang
Journal:Biomaterials(2016):12--26
Fiber-opticproteasesensorbasedonthedegradationofthingelatinfilms
Authors:BastienSchyrr,StéphanieBoder-Pasche,RéalIscher,RitaSmajda,GuyVoirin
Journal:SensingandBio-SensingResearch(2015):65--73
