| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 400/510 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | SmallMoleculeDetection DiagnosticMolecules |
| Related | RedoxEnzymes BiochemicalAssays |
1.Prepare500XAldeLight™Greenstocksolution:
Add20µLofDMSO(ComponentD)intotheAldeLight™Greenvial(ComponentA)tomake500Xstocksolution.
Note:TheunusedAldeLight™Greensolutionshouldbealiquoted,andstoredat-20oC(avoidlight).
2.PrepareAldeLight™Greenreactionmixture:
Add10µLof500XAldeLight™Green(fromStep1)into5mLofAssayBuffer(fromComponentB),mixwell.
Note:5mLofAldeLight™Greenreactionmixtureisenoughfor1plate.Thereactionmixtureisnotstable,andbestusedwithin2hours.
3.Prepareserialformaldehydestandard(0to300µM)solutions:
3.1 Add5µLof37.2%FormaldehydeStandard (ComponentD)into0.5mLofAssayBuffer(fromComponentB)tomake123mMstocksolution.
3.2 Add12.2μLof123mMFormaldehydeStandardSolution(fromStep3.1)into0.5mLofAssayBuffer(fromComponentB)tomake3mMstocksolution.
3.3 Take3mMFormaldehydeStandardSolution(fromStep3.2)toperform1:10,and1:3serialdilutionstoget 300,100,30,10,3,1,0.3,0.1,and0μMstandardformaldehydesolutions.
3.4 Addformaldehydestandardsandformaldehyde-containingtestsamplesintoa96-wellblacksolidmicroplateasdescribedinTables1and2
Table1.Layoutofformaldehydestandardsandtestsamplesinablacksolid96-wellmicroplate
BL | BL | TS | TS | …. | …. |
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FS1 | FS1 | …. | …. | …. | …. |
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FS2 | FS2 |
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FS3 | FS3 |
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FS4 | FS4 |
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FS5 | FS5 |
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FS6 | FS6 |
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FS7 | FS7 |
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Note:FS=FormaldehydeStandards,BL=BlankControl,TS=TestSamples.
Table2.Reagentcompositionforeachwell
FormaldehydeStandard | BlankControl | TestSample |
Serialdilutions*(50μL) | Assaybuffer:50μL | 50μL |
*Note:Addtheseriallydilutedformaldehydestandardsfrom0.1μMto100μMintowellsfromFS1toFS7induplicate.
4.Runformaldehydeassay:
4.1 Add50μLofAldeLight™Greenreactionmixtures(fromStep2)toeachwelloftheformaldehydestandard,blankcontrol,andtestsamples(seeStep3.4)tomakethetotalformaldehydeassayvolumeof100µL/well.
Note:For384-wellplateadd25μLoftestsampleand25μLofAldeLight™Greenreactionmixturesintoeachwell.
4.2 Incubatethereactionmixtureatroomtemperaturefor20to60minutes(protectedfromlight).
4.3 MonitorthefluorescenceincreaseatEx/Em=410/525nmusingafluorescenceplatereader.
| References&Citations |  CitationExplorer |
BIOLOGicalActivityofPeptide-conjugatedPolyionComplexMatricesConsistingofAlginateandChitosan
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Journal:PeptideScience(2016)
HepaticDeficiencyofAugmenterofLiverRegenerationExacerbatesAlcohol-InducedLiverInjuryandPromotesFibrosisinMice
Authors:SudhirKumar,JiangWang,RichaRani,ChandrashekharRGandhi
Journal:PloSone(2016):e0147864
Integratedself-assemblingdrugdeliverysystempossessingdualresponsiveandactivetargetingfororthotopicovariancancertheranostics
Authors:Chun-JuiLin,Chen-HsiangKuan,Li-WenWang,Hsi-ChinWu,YunchingChen,Chien-WenChang,Rih-YangHuang,Tzu-WeiWang
Journal:Biomaterials(2016):12--26
Fiber-opticproteasesensorbasedonthedegradationofthingelatinfilms
Authors:BastienSchyrr,StéphanieBoder-Pasche,RéalIscher,RitaSmajda,GuyVoirin
Journal:SensingandBio-SensingResearch(2015):65--73
