| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 420/None |
| MW | N/A |
| CAS# | 30931-67-0 |
| Solvent | N/A |
| Storage | R/D/L |
| Category | EnzymeDetection HorserADIshPeroxidase(HRP) |
| Related | BiochemicalAssays |
1. WarmReadiUse™ABTSSolutiontoroomtemperaturebeforeuse.
Note:Thereagentistobeusedassupplied,nodilutionisrequired.
2. WashtheassayplatefollowingtheincubationofHRP-labeledreagent.
3. Add100μLofReadiUse™ABTSSolutionintoeachwell.
4. Incubatetheplateatroomtemperaturefor15–30min.
Note:Theincubationtimevariesdependingontheassayconditions.
5. Measuretheabsorbancesignalat415+10nm(maximumat420nm)withanELISAmicroplatereader.
| References&Citations |  PrinterFriendlyVersion |
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2. ElbazJ,MosheM,ShlyahovskyB,WillnerI.(2009)Cooperativemulticomponentself-assemblyofnucleicacidstructuresfortheactivationofDNAzymecascades:aparadigmforDNAsensorsandaptasensors.Chemistry,15,3411.
3. FreemanR,SharonE,Tel-VeredR,WillnerI.(2009)Supramolecularcocaine-aptamercomplexesactivatebiocatalyticcascades.JAmChemSoc,131,5028.
4. PizzulL,CastilloMD,StenstromJ.(2009)Degradationofglyphosateandotherpesticidesbyligninolyticenzymes.Biodegradation.
5. vanDongenSF,NallaniM,CornelissenJJ,NolteRJ,vanHestJC.(2009)Athree-enzymecascadereactionthroughpositionalassemblyofenzymesinapolymersomenanoreactor.Chemistry,15,1107.
6. WilnerOI,ShimronS,WeizmannY,WangZG,WillnerI.(2009)Self-assemblyofenzymesonDNAscaffolds:enroutetobiocatalyticcascadesandthesynthesisofmetallicnanowires.NanoLett,9,2040.
7. LloberaA,CadarsoVJ,DarderM,DominguezC,Fernandez-SanchezC.(2008)Full-fieldphotonicbiosensorsbasedontunablebio-dopedsol-gelglasses.LabChip,8,1185.
8. OzyurekM,BektasogluB,GucluK,GungorN,ApakR.(2008)Simultaneoustotalantioxidantcapacityassayoflipophilicandhydrophilicantioxidantsinthesameacetone-watersolutioncontaining2%methyl-beta-cyclodextrinusingthecupricreducingantioxidantcapacity(CUPRAC)method.AnalChimActa,630,28.
9. RyanBJ,O"FagainC.(2008)EffectsofmutationsinthehelixGregionofhorseradishperoxidase.Biochimie,90,1414.
10. Duarte-VazquezMA,Garcia-PadillaS,Garcia-AlmendarezBE,WhitakerJR,RegaladoC.(2007)Broccoliprocessingwastesasasourceofperoxidase.JAgricFoodChem,55,10396.
