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AAT Bioquest/ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*/11012/100 ml
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 650/None |
| MW | 240.34 |
| CAS# | 54827-17-7 |
| Solvent | N/A |
| Storage | R/D/L |
| Category | EnzymeDetection HorserADIshPeroxidase(HRP) |
| Related | BiochemicalAssays |
1. WarmReadiUse™TMBSolutiontoroomtemperaturebeforeuse.
Note:Thereagentistobeusedassupplied,nodilutionisrequired.
2. WashtheassayplatefollowingtheincubationofHRP-labeledreagent.
3. Add100μLofReadiUse™TMBSolutionintoeachwell.
4. Incubatetheplateatroomtemperaturefor15–30minoruntilthedesiredcolordevelops.
Note:Theincubationtimevariesdependingontheassayconditions.
5. Measuretheabsorbancesignalat650nmwithanELISAmicroplatereader.
| References&Citations |  PrinterFriendlyVersion |
1. LiuY,ZhuG,YangJ,YuanA,ShenX.(2014)Peroxidase-LikeCatalyticActivityofAg3PO4NanocrystalsPreparedbyaColloidalRoute.PLoSOne,9,e109158.
2. JiangX,SunC,GuoY,NieG,XuL.(2014)Peroxidase-likeactivityofapoferritinpairedgoldclustersforglucosedetection.BiosensBioelectron,64C,165.
3. LiuQ,YangY,LiH,ZhuR,ShaoQ,YangS,XuJ.(2014)NiOnanoparticlesmodifiedwith5,10,15,20-tetrakis(4-carboxylpheyl)-porphyrin:PromisingperoxidasemimeticsforHOandglucosedetection.BiosensBioelectron,64C,147.
4. AumillerWM,Jr.,DavisBW,HatzakisE,KeatingCD.(2014)Interactionsofmacromolecularcrowdingagentsandcosoluteswithsmall-moleculesubstrates:effectonhorseradishperoxidaseactivitywithtwodifferentsubstrates.JPhysChemB,118,10624.
5. LiuQ,JiaQ,ZhuR,ShaoQ,WangD,CuiP,GeJ.(2014)5,10,15,20-tetrakis(4-carboxylphenyl)porphyrin-CdSnanocompositeswithintrinsicperoxidase-likeactivityforglucosecolorimetricdetection.MaterSciEngCMaterBiolAppl,42,177.
6. LinT,ZhongL,SongZ,GuoL,WuH,GuoQ,ChenY,FuF,ChenG.(2014)Visualdetectionofbloodglucosebasedonperoxidase-likeactivityofWS2nanosheets.BiosensBioelectron,62,302.
7. PanY,LiN,MuJ,ZhouR,XuY,CuiD,WangY,ZhaoM.(2014)BiogenicmagneticnanoparticlesfromBurkholderiasp.YN01exhibitingintrinsicperoxidase-likeactivityandtheirapplications.ApplMicrobiolBiotechnol.
8. MitraK,GhoshAB,SarkarA,SahaN,DuttaAK.(2014)Colorimetricestimationofhumanglucoselevelusinggamma-Fe(2)O(3)nanoparticles:aneasilyrecoverableeffectivemimicperoxidase.BiochemBiophysResCommun,451,30.
9. LiuQ,LiH,ZhaoQ,ZhuR,YangY,JiaQ,BianB,ZhuoL.(2014)Glucose-sensitivecolorimetricsensorbasedonperoxidasemimicsactivityofporphyrin-Fe3O4nanocomposites.MaterSciEngCMaterBiolAppl,41,142.
10. ChenX,TianX,SuB,HuangZ,OyamaM.(2014)Aunanoparticlesoncitrate-functionalizedgraphenenanosheetswithahighperoxidase-likeperformance.DaltonTrans,43,7449.
