| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | None/None |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F |
| Category | EnzymeDetection HorserADIshPeroxidase(HRP) |
| Related |
1. Make1XStopReagentbydilutionHRPReactionStoppingSolution20folds,e.g.,adding50µLofSignalGuard™HRPreactionstoppingsolutioninto950µLddH2O.
Note:Preparetheworkingsolutionsfreshlyasneeded,andavoidlight.
2. Atthedesiredstoppingtimepoint,add20µLof1XStopReagentsolutionper100µLvolumeineachmicroplatewell.
Note: Forotherreactionvolumes,adjusttheadditionof1XStopReagentproportionally(e.g.add5µLtoa25µLreactionvolume).The1Xstopreagentshouldbeaddedtoallwells,includinganyreagentcontrolswithoutHRP.Thetime-dependentfluorescencesignalincreasewillterminateimmediatelyandthefluorescencesignallevelshouldremainstableforatleast5hours.
Figure1.TheApplicationofHRPReactionStopSolutioninHRP-coupledglucosedetectionreaction.Twoparallelreactionscontaining15µMGlucosewereinitiatedbyadding50µLassaymixturecontaining:0.5mU/mLHRP,Amplex®Red,and0.5mU/mlGlucoseOxidase.Reactionswereincubatedatroomtemperaturefor5minsandthen20µL1XStopReagentwasaddedtoonereaction(A),and20µLddH2Ototheotherreaction(B).TheplotsdemonstratedthatthereactioniscompletelyinhibitedbySignalGuard™HRPReactionStoppingSolution.
| References&Citations |  CitationExplorer |
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Journal:ClinicalCancerResearch(2011):4484--4493
