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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT Bioquest/AA-dUTP [Aminoallyl dUTP sodium salt] *4 mM in Tris Buffer (pH 7.5)* *CAS 936327-10-5*/17005/2.5 umoles
商品详细AAT Bioquest/AA-dUTP [Aminoallyl dUTP sodium salt] *4 mM in Tris Buffer (pH 7.5)* *CAS 936327-10-5*/17005/2.5 umoles
AAT Bioquest/AA-dUTP [Aminoallyl dUTP sodium salt] *4 mM in Tris Buffer (pH 7.5)* *CAS 936327-10-5*/17005/2.5 umoles
AAT Bioquest/AA-dUTP [Aminoallyl dUTP sodium salt] *4 mM in Tris Buffer (pH 7.5)* *CAS 936327-10-5*/17005/2.5 umoles
商品编号: 17005
品牌: aatbio
市场价: ¥3900.00
美元价: 2340.00
产地: 美国(厂家直采)
公司:
产品分类: 其他生物染料
公司分类: Other_biological_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)None/None
MW589.17
CAS#936327-10-5
SolventWater
StorageF/D/L
CategoryNucleicAcidDetection
DNADetection
RelatedPolymerases
BiochemicalAssays
Theamine-modifieddeoxyuridine5"-triphosphates(suchasaminoallyl-dUTP)canbeusedtoproduceamine-containingDNAbyconventionalenzymaticincorporationmethodssuchasreversetranscription,nicktranslation,randomprimedlabeling,orPCR.AminoallyldUTPcanbereADIlyincorporatedintoDNAthroughtheconventionalenzymaticincorporationtechniques.Theresultingamine-modifiednucleicacidscanthenbelabeledusinganyofamine-reactivefluorescentdyes,biotinsandotheramine-reactivereagents.Theaminoallyl-modifiednucleotidescanbeincorporatedtoextremelyhighandconsistentlevelscomparedtothetag-labeleduridinetriphosphatesthatgenerallyhavehigherstereo-hindrance.Subsequentreactionoftheamine-modifiednucleicacidwithanexcessofamine-reactivereagentachievescorrespondinglyhighandconsistentlabelingefficiencies,regardlessofthelabelingreagentchosen.Thistwo-steplabelingmethodalsoeliminatestheneedtooptimizeanenzymaticreactiontoaccommodatedifferentdye-modifiednucleotides,whichmayincorporateatverydifferentrates.ThislabelingmethodiswidelyusedforbothFISHprobesandmicroarray-basedexperiments.
References&Citations
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1.   CoxWG,SingerVL.(2004)FluorescentDNAhybridizationprobepreparationusingaminemodificationandreactivedyecoupling.Biotechniques,36,114.

2.   WenJK,ZhangXE,ChengZ,LiuH,ZhouYF,ZhangZP,YangJH,DengJY.(2004)AvisualDNAchipforsimultaneousdetectionofhepatitisBvirus,hepatitisCvirusandhumanimmunodeficiencyvirustype-1.BiosensBioelectron,19,685.

3.   SchoetzauT,LangnerJ,MoyroudE,RoehlI,VonhoffS,KlussmannS.(2003)Aminomodifiednucleobases:functionalizednucleosidetriphosphatesapplicableforSELEX.BioconjugChem,14,919.

4.   WooddellCI,BurgessRR.(2000)TopologyofyeastRNApolymeraseIIsubunitsintranscriptionelongationcomplexesstudiedbyphotoaffinitycross-linking.Biochemistry,39,13405.

5.   NimmakayaluM,HenegariuO,WardDC,Bray-WardP.(2000)Simplemethodforpreparationoffluor/hapten-labeleddUTP.Biotechniques,28,518.

6.   DuplaaC,CouffinhalT,LabatL,MoreauC,LamaziereJM,BonnetJ.(1993)QuantitativeanalysisofpolymerasechainreactionproductsusingbiotinylateddUTPincorporation.AnalBiochem,212,229.

7.   UrabeT,SanoK,TannoM,MizoguchiJ,OtaniM,LeeMH,TakasakiT,KusakabeH,ImagawaDT,NakaiM.(1992)Anon-radioisotopicreversetranscriptaseassayusingbiotin-11-deoxyuridinetriphosphateonprimer-immobilizedmicrotiterplates.JVirolMethods,40,145.

8.   DawsonBA,HermanT,HaasAL,LoughJ.(1991)Affinityisolationofactivemurineerythroleukemiacellchromatin:uniformdistributionofubiquitinatedhistoneH2Abetweenactiveandinactivefractions.JCellBiochem,46,166.

9.   MuhleggerK,HuberE,vonderEltzH,RugerR,KesslerC.(1990)Non-radioactivelabelinganddetectionofnucleicacids.IV.Synthesisandpropertiesofdigoxigenin-modified2"-deoxyuridine-5"-triphosphatesandaphotoactivatableanalogofdigoxigenin(photodigoxigenin).BiolChemHoppeSeyler,371,953.

10.   DawsonBA,HermanT,LoughJ.(1989)AffinityisolationoftranscriptionallyactivemurineerythroleukemiacellDNAusingacleavablebiotinylatednucleotideanalog.JBiolChem,264,12830.


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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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