| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 405/None |
| MW | 724.37 |
| CAS# | N/A |
| Solvent | Water |
| Storage | F/D/L |
| Category | EnzymeDetection Phosphodiesterases |
| Related | BiochemicalAssays |
ThefollowingrecommendedprocedurecanbeadaptedformeasuringPARP-1,tankyrase-1,andVPARPactivitiesandtheirinhibitors.Theoptimumconditionsmustbedeterminedexperimentallyforeachtest.
1. Make5-10mMstocksolutioninH2O.Thestocksolutionshouldbeusedpromptly.
Note:Anyunusedsolutionshouldbealiquotedandrefrozenat-20oC.Avoidrepeatedfreeze-thawcyclesandkeepfromlight.
- Takeonevialout,andmake0.25mMassaysolutionbydilutingthestocksolutionwithassaybuffer(50mMTris,10mMMgCl2,pH8.0).
- Add0.01mL/wellofsamplesolutioninto0.09mL/wellassaysolution(fromStep2)tomakeafinalvolumeof0.1mLina96-wellclearplate.
- Monitortheplateusinganabsorbancemicroplatereaderat405nm.
| References&Citations |  PrinterFriendlyVersion |
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2. MeliE,PangalloM,BarontiR,ChiarugiA,CozziA,Pellegrini-GiampietroDE,MoroniF.(2003)Poly(ADP-ribose)polymeraseasakeyplayerinexcitotoxicityandpost-ischemicbraindamage.ToxicolLett,139,153.
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5. GallettoR,RajendranS,BujalowskiW.(2000)InteractionsofnucleotidecofactorswiththeEscherichiacolireplicationfactorDnaCprotein.Biochemistry,39,12959.
6. DukeAM,SteeleDS.(1998)EffectsofcaffeineandadeninenucleotidesonCa2+releasebythesarcoplasmicreticuluminsaponin-permeABIlizedfrogskeletalmusclefibres.JPhysiol,513(Pt1),43.
7. SchroederP,JustI,AktoriesK.(1994)AdeninenucleotidesregulateADP-ribosylationofmembrane-boundactinandactin-bindingtomembranes.EurJCellBiol,63,3.
8. BarrFA,LeyteA,MollnerS,PfeufferT,ToozeSA,HuttnerWB.(1991)TrimericG-proteinsofthetrans-Golginetworkareinvolvedintheformationofconstitutivesecretoryvesiclesandimmaturesecretorygranules.FEBSLett,294,239.
9. MignacoJ,ScofanoHM,BarrabinH.(1990)InhibitionandlabelingoftheCa2(+)-ATPasefromsarcoplasmicreticulumbyperiodateoxidizedATP.BiochimBiophysActa,1039,305.
10. RamanarayananM,SrinivasanPR.(1976)FurtherstudiesontheisolationandpropertiesofpolyriboadenylatepolymerasefromEscherichiacoliPR7(RNaseI-,pnp).JBiolChem,251,6274.
