| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | None/560 |
| MW | 868.03 |
| CAS# | N/A |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | EnzymeDetection PeptidasesandProteases |
| Related | BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1. Preparea5to10mMstocksolutioninDMSO.
2. Preparea2Xproteasomesubstrate(20to50µM)assaysolutionasthefollowing:
25to50µLsubstratestocksolution(10mM)
100µLDTT(1M)
400µLEDTA(100mM)
10mLHepesBuffer(25mM),pH=7.4
3. Mixequalvolumeoftheprotesomestandardsorsampleswith2Xfluorescentproteasomesubstrateassaysolution(fromStep1),andincubatethesolutionsatroomtemperatureforatleast1hour.
4. Monitorthefluorescenceusefluorescentmicroplatereaders.
| References&Citations |  CitationExplorer |
Diabetogenicagentalloxanisaproteasomeinhibitor
Authors:WenjuanZhou,LinglingWei,TingXiao,ChunyouLai,MinPeng,LingliXu,XiangweiLuo,ShaopingDeng,FengxueZhang
Journal:BiochemicalandBiophysicalResearchCommunications(2017):400--406
DelineationofmolecularpathwaysinvolvedincardiomyopathiescausedbytroponinTmutations
Authors:JenniferEGilda,XianyinLai,FrankAWitzmann,AldrinVGomes
Journal:Molecular&CellularProteomics(2016):1962--1981
Diclofenacinducesproteasomeandmitochondrialdysfunctioninmurinecardiomyocytesandhearts
Authors:RajeshwaryGhosh,SumantaKGoswami,LuisFelipeBBFeitoza,BruceHammock,AldrinVGomes
Journal:InternationalJournalofCardiology(2016):923--935
Advanced-glycation-end-product-inducedformationofimmunoproteasomes:involvementofRAGEandJak2/STAT1
Authors:StefanieGrimm,ChristianeOtt,MelanieHörlacher,DanielaWeber,AnnikaHöhn,TilmanGrune
Journal:BiochemicalJournal(2012):127--139
