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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT生物探索/阅读与贸易;TCEP去除缓冲液/5540/1毫升
商品详细AAT生物探索/阅读与贸易;TCEP去除缓冲液/5540/1毫升
AAT生物探索/阅读与贸易;TCEP去除缓冲液/5540/1毫升
AAT生物探索/阅读与贸易;TCEP去除缓冲液/5540/1毫升
商品编号: 5540
品牌: aatbio
市场价: ¥3900.00
美元价: 2340.00
产地: 美国(厂家直采)
公司:
产品分类: 其他生物染料
公司分类: Other_biological_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)None/None
MWN/A
CAS#N/A
SolventN/A
StorageR
CategoryProteinBiochemistry
Generalproteins
Related
TCEPisacommonreducingreagentwidelyusedtobreakdisulfidebondswithinandbetweenproteins.Comparedtotheothertwomostcommonreducingagents,i.e.,dithiothreitol(DTT)and2-mercaptoethanol,TCEPhastheadvantagesofbeingodorless,amorepowerfulreducingagent,anirreversIBLereducingagent,morehydrophilic,andmoreresistanttooxidationinair.TCEPisalsousedinthetissuehomogenizationprocessforRNAisolation.However,therearenumerousreportsthatTCEPisnotcompatiblewithdownstreamassaysthatoftenhavecomponentsreactivetoTCEP.OurReADIUse™TCEPremovingbuffercanbeeffectivelyusedforeliminatingresidualTCEP.Itisapreformatted2MsolutionthatcanbesimplyaddedtoasolutionthatcontainsresidualTCEP.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. WarmupReadiLink™TCEPremovalbufferatroomtemperaturebeforeuse.

  2. EstimatetheTCEPamounttoberemoved.

  3. AddtheReadiLink™TCEPremovalbuffertoasolutionthatcontainsresidualTCEPatmolarratioof100:1TCEPRemovalBuffer/TCEP(toberemoved)

  4. Incubatethesolution(fromStep2)atroomtemperaturefrom30to60minutes.

  5. Thesolutionisnowreadytousefordesireddownstreamassays.
References&Citations
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  1. ShuT,WangJ,SuL,ZhangX.(2016)ChemicalEtchingofBovineSerumAlbumin-ProtectedAu25NanoclustersforLabel-FreeandSeparation-FreeRatiometricFluorescentDetectionofTris(2-carboxyethyl)phosphine.AnalChem,88,11193.
  2. EutiEM,Velez-RomeroP,LeivaEP,MacagnoVA,Paredes-OliveraPA,PatritoEM,ComettoFP.(2016)TheRoleofTris(2-carboxyethyl)phosphineReducingAgentintheControlledFormationofalpha,omega-AlkanedithiolsMonolayersonAu(111)withMonocoordinatedandBicoordinatedConfigurations.Langmuir,32,9428.
  3. CornellC,KaranjitA,ChenY,JacobsonF.(2016)Ahigh-throughputhydrophilicinteractionliquidchromatographycoupledwithachargedaerosoldetectormethodtoassesstrisulfidesinIgG1monoclonalantibodiesusingtris(2-carboxyethyl)phosphinereactionproducts:Tris(2-carboxyethyl)phosphine-oxideandtris(2-carboxyethyl)phosphine-sulfide.JChromatogrA,1457,107.
  4. PruchnikH,LisT,LatochaM,ZielinskaA,PruchnikFP.(2016)Palladium(II)complexeswithtris(2-carboxyethyl)phosphine,structure,reactionsandcytostaticactivity.JInorgBiochem,156,14.
  5. JongbergS,LundMN,OtteJ.(2015)Dissociationandreductionofcovalentbeta-lactoglobulin-quinoneadductsbydithiothreitol,tris(2-carboxyethyl)phosphine,orsodiumsulfite.AnalBiochem,478,40.
  6. Shriver-LakeLC,NorthSH,RoweTaittC.(2013)Lossofcationicpeptideswithagarosegel-immobilizedtris[2-carboxyethyl]phosphine(TCEP).Biotechniques,55,292.
  7. ParkJB,BaeSH,JangSM,NohWJ,HongJH,YoonKD,KangHC,BaeSK.(2013)Directmeasurementofactivethiolmetabolitelevelsofclopidogrelinhumanplasmausingtris(2-carboxyethyl)phosphineasareducingagentbyLC-MS/MS.JSepSci,36,2306.
  8. MirallesG,VerdieP,PugetK,MaurrasA,MartinezJ,SubraG.(2013)Microwave-mediatedreductionofdisulfidebridgeswithsupported(tris(2-carboxyethyl)phosphine)asresin-boundreducingagent.ACSCombSci,15,169.
  9. ChenS,JiangH,WeiK,LiuY.(2013)Tris-(2-carboxyethyl)phosphinesignificantlypromotesthereactionofcisplatinwithSp1zincfingerprotein.ChemCommun(Camb),49,1226.
  10. TzanavarasPD,MitaniC,AnthemidisA,ThemelisDG.(2012)On-linecleavageofdisulfidebondsbysolubleandimmobilizedtris-(2-carboxyethyl)phosphineusingsequentialinjectionanalysis.Talanta,96,21.

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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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