| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 620/None |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | SmallMoleculeDetection MetalIons |
| Related | BiochemicalAssays |
1. ZnCl2standardsandtestsamplepreparations:
1.1 Add10μLof100mMZnCl2Standardsolution(ComponentC)into990µLAssayBuffer(Component
B)toget1mMZnCl2standardsolution.
1.2 Add100μLof1mMZincStandardsolution(ComponentC)to990µLAssayBuffer(ComponentB)to
get100µMZnCl2standardsolution.
1.3 Take300μLof100µMZnCl2standardsolution(fromStep1.2)toperform1:2serialdilutionstoget
50,25,12.5,6.25,3.13,1.56and0µMseriallydilutedZnCl2standards.
1.4 Dilutethetestsampleto5-100µMrangewithAssayBuffer(ComponentB).
1.5 Add50μLofZnCl2standards,dilutedsampleandcontrolintoeachwellasshowninTable1and2.
Table1.LayoutofZnCl2standardsandtestsamplesinaclearbottom96-wellmicroplate
BL | BL | TS | TS | …. | …. |
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Zn1 | Zn1 | …. | …. | …. | …. |
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Zn2 | Zn2 |
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Zn3 | Zn3 |
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Zn4 | Zn4 |
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Zn5 | Zn5 |
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Zn6 | Zn6 |
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Zn7 | Zn7 |
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Note:Zn=ZincStandards,BL=BlankControl,TS=TestSamples.
Table2.Reagentcompositionforeachwell
ZincStandard | BlankControl | TestSample |
Serialdilutions*:50μL | AssayBuffer:50μL | 50μL |
*Note:AddtheseriallydilutedZnCl2standardsfrom100µMto1.56µMintowellsfromZn1toZn7induplicate.
2. RunZincassay:
2.1 Add25μLof Zn-620™(ComponentA)into5mLAssayBuffer(ComponentB)tomakeZnassay
mixture.Add50μLZnassaymixturetoeachwellofZnCl2standard,blankcontrol,andtestsamples(seeStep1.5,Table1and2)tomakethetotalZnCl2assayvolumeof100µL/well.
Note: Fora384-wellplate,add25μLofsamplesand25μLofassayreactionmixtureintoeachwell.
2.2 Incubatethereactionfor5-10minutesatroomtemperature,protectedfromlight.
2.3 Monitortheabsorbanceincreasewithaabsorbanceplatereaderat610nmand480nm.
| References&Citations |  CitationExplorer |
Afteroxidation,zincnanoparticleslosetheirABIlitytoenhanceresponsestoodorants
Authors:SamanthaHagerty,YasmineDaniels,MelissaSingletary,OlegPustovyy,LudmilaGloba,WilliamAMacCrehan,ShinMuramoto,GheorgheStan,JuneWLau,EdwardEMorrison
Journal:BioMetals(2016):1--14
