| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 492/515 |
| MW | ~800 |
| CAS# | N/A |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | EnzymeDetection Phosphodiesterases |
| Related | cAMPGPCRAssays BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1. Makea1mMstocksolutionbyadding500µLofDMSOintothevialof0.5umolFAM-cAMPPDEIVsubstrate.
2. Make2XFAM-Cyclic-3’,5’-AMPPDEIVsubstrateassaysolutionbydilute1mMFAM-Cyclic-3’,5’-AMPPDEIV substratestocksolutionintoyourPDEbuffer(suchas10mMTris-HCl,pH7.4,10mMMgCl2,1mMMnCl2)tomakea200-400nMsolution.Makeonlysufficientquantityneededfortheassay;storeremainingstocksolutioninaliquotsat-20°C.
3. MixequalvolumeofthePDEIVstandardsorsampleswith2XFAM-Cyclic-3’,5’-AMPPDEIVsubstrateassaysolution,andincubateatroomtemperatureforatleast1hour.
4. MonitorthefluorescencepolarizationatEx/Em=490/525nm.
| References&Citations |  PrinterFriendlyVersion |
1. AlonsoGD,SchoijetAC,TorresHN,FlawiaMM.(2006)TcPDE4,anovelmembrane-associatedcAMP-specificphosphodiesterasefromTrypanosomacruzi.MolBiochemParasitol,145,40.
2. BaderS,KortholtA,SnippeH,VanHaastertPJ.(2006)DdPDE4,anovelcAMP-specificphosphodiesteraseatthesurfaceofdictyosteliumcells.JBiolChem,281,20018.
3. BolgerGB,BaillieGS,LiX,LynchMJ,HerzykP,MohamedA,MitchellLH,McCahillA,HundsruckerC,KlussmannE,AdamsDR,HouslayMD.(2006)Scanningpeptidearrayanalysesidentifyoverlappingbindingsitesforthesignallingscaffoldproteins,beta-arrestinandRACK1,incAMP-specificphosphodiesterasePDE4D5.BiochemJ,398,23.
4. CharlieNK,ThomureAM,SchadeMA,MillerKG.(2006)TheDuncecAMPphosphodiesterasePDE-4negativelyregulatesGalpha(s)-dependentandGalpha(s)-independentcAMPpoolsintheCaenorhaBDitiseleganssynapticsignalingnetwork.Genetics,173,111.
5. Diaz-BenjumeaR,LaxmanS,HindsTR,BeavoJA,RasconA.(2006)CharacterizationofanovelcAMP-binding,cAMP-specificcyclicnucleotidephosphodiesterase(TcrPDEB1)fromTrypanosomacruzi.BiochemJ,399,305.
6. Diaz-BenjumeaR,LaxmanS,HindsTR,BeavoJA,RasconA.(2006)CharacterizationofanovelcAMP-binding,cAMP-specificcyclicnucleotidephosphodiesterase(TcrPDEB1)fromTrypanosomacruzi.BiochemJ,399,305.
7. Gross-LangenhoffM,HofbauerK,WeberJ,SchultzA,SchultzJE.(2006)cAMPisaligandforthetandemGAFdomainofhumanphosphodiesterase10andcGMPforthetandemGAFdomainofphosphodiesterase11.JBiolChem,281,2841.
8. HanP,SonatiP,RubinC,MichaeliT.(2006)PDE7A1,acAMP-specificphosphodiesterase,inhibitscAMP-dependentproteinkinasebyadirectinteractionwithC.JBiolChem,281,15050.
9. HillEV,SheppardCL,CheungYF,GallI,KrauseE,HouslayMD.(2006)Oxidativestressemploysphosphatidylinositol3-kinaseandERKsignallingpathwaystoactivatecAMPphosphodiesterase-4D3(PDE4D3)throughmulti-sitephosphorylationatSer239andSer579.CellSignal,18,2056.
10. HsuHT,ChangYC,ChiuYN,LiuCL,ChangKJ,GuoIC.(2006)Leptininterfereswithadrenocorticotropin/3",5"-cyclicadenosinemonophosphate(cAMP)signaling,possiblythroughaJanuskinase2-phosphatidylinositol3-kinase/Akt-phosphodiesterase3-cAMPpathway,todown-regulatecholesterolside-chaincleavagecytochromeP450enzymeinhumanadrenocorticalNCI-H295cellline.JClinEndocrinolMetab,91,2761.
