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当前位置: 首页 > 产品中心 > Fluorescent_dyes > AAT Bioquest/TAMRA-cAMP PDE IV substrate *Red Fluorescence*/13603/0.5 umol
商品详细AAT Bioquest/TAMRA-cAMP PDE IV substrate *Red Fluorescence*/13603/0.5 umol
AAT Bioquest/TAMRA-cAMP PDE IV substrate *Red Fluorescence*/13603/0.5 umol
AAT Bioquest/TAMRA-cAMP PDE IV substrate *Red Fluorescence*/13603/0.5 umol
商品编号: 13603
品牌: aatbio
市场价: ¥85560.00
美元价: 51336.00
产地: 美国(厂家直采)
公司:
产品分类: 荧光染料
公司分类: Fluorescent_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)544/575
MW~900
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryEnzymeDetection
Phosphodiesterases
RelatedcAMPGPCRAssays
BiochemicalAssays
ThisredcAMPderivativeisaspecificsubstrateforphosphodiesterase(PDE)IV.ItcanbeusedforassayingPDEIVactivitiesorscreeningPDEIVinhibitorsincombinationwithanti-cAMPantibodyinaFRETreadoutorFPformat.PDEisagroupofenzymesthatdegradethesecondmessengermolecules:cyclicnucleotidescAMPandcGMP.Theyregulatethelocalization,duration,andamplitudeofcyclicnucleotidesignalingwithinsubcellulardomains.PDEsarethereforeimportantregulatorsofsignaltransductionmediatedbythesesecondmessengermolecules.PDEenzymesareoftentargetsforpharmacologicalinhibitionduetotheiruniquetissuedistribution,structuralandfunctionalproperties.InhibitorsofPDEcanprolongorenhancetheeffectsofphysiologicalprocessesmediatedbycAMPorcGMPbyinhibitionoftheirdegradationbyPDE.PDEinhibitorshavebeenidentifiedasnewpotentialtherapeuticsinareassuchaspulmonaryarterialhypertension,coronaryheartdisease,dementia,depressionandschizophrenia.
SpectrumAdvancedSpectrumViewer

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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.      Makea1mMstocksolutionbyadding500µLofDMSOintothevialof0.5µmolTAMRA-cAMPPDEIVsubstrate.

 

2.      Make2XTAMRA-Cyclic-3’,5’-AMPPDEIVsubstrateassaysolutionbydilute1mMTAMRA-Cyclic-3’,5’-AMPPDEIV substratestocksolutionintoyourPDEbuffer(suchas10mMTris-HCl,pH7.4,10mMMgCl2,1mMMnCl2)tomakea200-400nMsolution.Makeonlysufficientquantityneededfortheassay;storeremainingstocksolutioninaliquotsat-20°C. 

 

3.      MixequalvolumeofthePDEIVstandardsorsampleswith2XTAMRA-Cyclic-3’,5’-AMPPDEIVsubstrateassaysolution,andincubateatroomtemperatureforatleast1hour.

 

4.      MonitorthefluorescencepolarizationatEx/Em=540/590nm(cutoff570nm).

References&Citations
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1.   AlonsoGD,SchoijetAC,TorresHN,FlawiaMM.(2006)TcPDE4,anovelmembrane-associatedcAMP-specificphosphodiesterasefromTrypanosomacruzi.MolBiochemParasitol,145,40.

2.   BaderS,KortholtA,SnippeH,VanHaastertPJ.(2006)DdPDE4,anovelcAMP-specificphosphodiesteraseatthesurfaceofdictyosteliumcells.JBiolChem,281,20018.

3.   BolgerGB,BaillieGS,LiX,LynchMJ,HerzykP,MohamedA,MitchellLH,McCahillA,HundsruckerC,KlussmannE,AdamsDR,HouslayMD.(2006)Scanningpeptidearrayanalysesidentifyoverlappingbindingsitesforthesignallingscaffoldproteins,beta-arrestinandRACK1,incAMP-specificphosphodiesterasePDE4D5.BiochemJ,398,23.

4.   CharlieNK,ThomureAM,SchadeMA,MillerKG.(2006)TheDuncecAMPphosphodiesterasePDE-4negativelyregulatesGalpha(s)-dependentandGalpha(s)-independentcAMPpoolsintheCaenorhaBDitiseleganssynapticsignalingnetwork.Genetics,173,111.

5.   Diaz-BenjumeaR,LaxmanS,HindsTR,BeavoJA,RasconA.(2006)CharacterizationofanovelcAMP-binding,cAMP-specificcyclicnucleotidephosphodiesterase(TcrPDEB1)fromTrypanosomacruzi.BiochemJ,399,305.

6.   Diaz-BenjumeaR,LaxmanS,HindsTR,BeavoJA,RasconA.(2006)CharacterizationofanovelcAMP-binding,cAMP-specificcyclicnucleotidephosphodiesterase(TcrPDEB1)fromTrypanosomacruzi.BiochemJ,399,305.

7.   Gross-LangenhoffM,HofbauerK,WeberJ,SchultzA,SchultzJE.(2006)cAMPisaligandforthetandemGAFdomainofhumanphosphodiesterase10andcGMPforthetandemGAFdomainofphosphodiesterase11.JBiolChem,281,2841.

8.   HanP,SonatiP,RubinC,MichaeliT.(2006)PDE7A1,acAMP-specificphosphodiesterase,inhibitscAMP-dependentproteinkinasebyadirectinteractionwithC.JBiolChem,281,15050.

9.   HillEV,SheppardCL,CheungYF,GallI,KrauseE,HouslayMD.(2006)Oxidativestressemploysphosphatidylinositol3-kinaseandERKsignallingpathwaystoactivatecAMPphosphodiesterase-4D3(PDE4D3)throughmulti-sitephosphorylationatSer239andSer579.CellSignal,18,2056.

10.   HsuHT,ChangYC,ChiuYN,LiuCL,ChangKJ,GuoIC.(2006)Leptininterfereswithadrenocorticotropin/3",5"-cyclicadenosinemonophosphate(cAMP)signaling,possiblythroughaJanuskinase2-phosphatidylinositol3-kinase/Akt-phosphodiesterase3-cAMPpathway,todown-regulatecholesterolside-chaincleavagecytochromeP450enzymeinhumanadrenocorticalNCI-H295cellline.JClinEndocrinolMetab,91,2761.


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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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