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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT Bioquest/Cy3酪酰胺/11065/1 mg
商品详细AAT Bioquest/Cy3酪酰胺/11065/1 mg
AAT Bioquest/Cy3酪酰胺/11065/1 mg
AAT Bioquest/Cy3酪酰胺/11065/1 mg
商品编号: 11065
品牌: aatbio
市场价: ¥27560.00
美元价: 16536.00
产地: 美国(厂家直采)
公司:
产品分类: 其他生物染料
公司分类: Other_biological_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)555/565
MW863.96
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryEnzymeDetection
HorserADIshPeroxidase(HRP)
Related
Formanyimmunohistochemical(IHC)applications,thetraditionalenzymaticamplificationproceduresaresufficientforachievingadequateantigendetection.However,severalfactorslimitthesensitivityandutilityoftheseprocedures.Tyramidesignalamplification(TSA)hasproventobeaparticularlyversatileandpowerfulenzymeamplificationtechniquewithimprovedassaysensitivity.TSAisbasedontheABIlityofHRP,inthepresenceoflowconcentrationsofhydrogenperoxide,toconvertlabeledtyramine-containingsubstrateintoanoxidized,highlyreactivefreeradicalthatcancovalentlybindtotyrosineresiduesatorneartheHRP.ToachievemaximalIHCdetection,tyramineisprelabeledwithafluorophore.Thesignalamplificationconferredbytheturnoverofmultipletyramidesubstratesperperoxidaselabeltranslatesultrasensitivedetectionoflow-abundancetargetsandtheuseofsmalleramountsofantibodiesandhybridizationprobes.Inimmunohistochemicalapplications,sensitivityenhancementsderivedfromTSAmethodallowprimaryantibodydilutionstobeincreasedtoreducenonspecificbackgroundsignals,andcanovercomeweakimmunolabelingcausedbysuboptimalfixationproceduresorlowlevelsoftargetexpression.Cy3tyramidecontainsthebrightCy3thatcanbereadilydetectedwiththestandardCy3filterset.
SpectrumAdvancedSpectrumViewer

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References&Citations
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1.   FagetL,HnaskoTS.(2015)TyramideSignalAmplificationforImmunofluorescentEnhancement.MethodsMolBiol,1318,161.

2.   TrangNT,HiraiT,NganPH,LanNT,FukeN,ToyamaK,YamamotoT,YamaguchiR.(2015)EnhanceddetectionofPorcinereproductiveandrespiratorysyndromevirusinfixedtissuesbyinsituhybridizationfollowingtyramidesignalamplification.JVetDiagnInvest,27,326.

3.   StackEC,WangC,RomanKA,HoytCC.(2014)Multiplexedimmunohistochemistry,imaging,andquantitation:areview,withanassessmentofTyramidesignalamplification,multispectralimagingandmultiplexanalysis.Methods,70,46.

4.   GarriguesHJ,RubinchikovaYE,RoseTM.(2014)KSHVcellattachmentsitesrevealedbyultrasensitivetyramidesignalamplification(TSA)localizetomembranemicrodomainsthatareup-regulatedonmitoticcells.Virology,452-453,75.

5.   AydinM,HerzigGP,JeongKC,DuniganS,ShahP,AhnS.(2014)RapidandsensitivedetectionofEscherichiacoliO157:H7inmilkandgroundbeefusingmagneticbead-basedimmunoassaycoupledwithtyramidesignalamplification.JFoodProt,77,100.

6.   LauterG,SollI,HauptmannG.(2014)Sensitivewhole-mountfluorescentinsituhybridizationinzebrafishusingenhancedtyramidesignalamplification.MethodsMolBiol,1082,175.

7.   ZhaoC,EisingerB,GammieSC.(2013)CharacterizationofGABAergicneuronsinthemouselateralseptum:adoublefluorescenceinsituhybridizationandimmunohistochemicalstudyusingtyramidesignalamplification.PLoSOne,8,e73750.

8.   DraberovaE,StegurovaL,SulimenkoV,HajkovaZ,DraberP.(2013)Quantificationofalpha-tubulinisotypesbysandwichELISAwithsignalamplificationthroughbiotinyl-tyramideorimmuno-PCR.JImmunolMethods,395,63.

9.   YuanL,XuL,LiuS.(2012)Integratedtyramideandpolymerization-assistedsignalamplificationforahighly-sensitiveimmunoassay.AnalChem,84,10737.

10.   GongH,CradduckM,CheungL,OliveDM.(2012)Developmentofanear-infraredfluorescenceELISAmethodusingtyramidesignalamplification.AnalBiochem,426,27.


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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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