4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
aatbio(优势品牌)
主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Routine_cell_strain > AAT Bioquest/Screen Quest™CHO-Gqs嵌合体细胞系/38104/株
商品详细AAT Bioquest/Screen Quest™CHO-Gqs嵌合体细胞系/38104/株
AAT Bioquest/Screen Quest™CHO-Gqs嵌合体细胞系/38104/株
AAT Bioquest/Screen Quest™CHO-Gqs嵌合体细胞系/38104/株
商品编号: 38104
品牌: aatbio
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 常规细胞株
公司分类: Routine_cell_strain
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)None/None
MWN/A
CAS#N/A
SolventN/A
StorageN2(L)
CategoryGPCR
cAMPGPCRAssays
Related
ScreenQuest™celllinesareaseriesofcellsthathavebeensuccessfullyusedindrugdiscoveryandscreeningenvironmentsforstudyingG-protein-coupledreceptors(GPCR)thatdonotconventionallycouplethroughintracellularcalcium.IthavebeeneffectivelyusedwiththeFLIPR,FDSSSystemsinconjunctionwithnon-Gqcoupledmembersofmanyreceptorssuchaschemokine,SEROtonin,glutamate,dopamine,opoid,vasopressinand?-andß-adrenoceptorreceptorfamilies.Over60%oftheknownG-protein-coupledreceptorssignalthroughpathwaysotherthanGqwhichleadtoanincreaseinintracellularcalcium,andasgenomicsrevealsmoreG-protein-coupledreceptortargetsthistrendcontinuestoincrease.ScreenQuest™celllinesareusedforinvestigatingG-protein-coupledreceptors(GPCR)thatdonotconventionallycouplethroughintracellularcalcium.ScreenQuest™celllinesarebasedonaseriesofG-proteinchimeras,includingthepromiscuousG-protein,G?16.ThechimerasconsistofthealphasubunitofaGq-proteincomplexwhose5carboxy-terminalaminoacidshavebeenreplacedwiththosefromoneoftheotherG-proteins(eitherG?s,G?i,G?o,orG?z).TheseaminoacidsareresponsIBLeforthecouplingofthereceptortoitsG-protein.Co-expressionofthesechimeraswithspecificnon-Gq-coupledreceptorswhichnormallyactthroughthecAMPpathwaymayresultinthegenerationofanintracellularcalciumsignaluponreceptorstimulation.ScreenQuest™CHO-GqscelllineisCHO-K1cellsstablytransfectedwiththechimericGqsalphasubunitprotein.WhenusedasahostcellfortransfectionexpressionofGs-coupledreceptors,theconstitutivelyexpressedGqsproteininthecellsallowsthetransfectedreceptorwhichnormallyactthroughthecAMPpathway,tocoupletoGqsignaltransductionandmobilizedintracellularcalcium.Activationofthespecificnon-Gq-coupledreceptorsinthesecellsbyspecificligandscanbedetectedusingcalciumsensitivedyessuchasCal-520AM,Fluo-8AM,orFluo-4AM.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. CellDensityandStorage
    Thecellsarefrozenatadensityof2X106cellsin90%fetalbovineserumand10%(v/v)DMSO.ToinsurethehighestlevelofviABIlity,thawthevialandinitiatethecultureassoonaspossibleuponreceipt.Cellsmustbestoredinliquidnitrogenifnotimmediatelyprocesseduponreceipt.
  2. CellCultureMedium
    Basalmedium:Ham’sF12culturemediumcontaining10%fetalbovineserum,2mML-glutamine,100units/mLpenicillin,100µg/mLstreptomycin(Basalmedium).
    SelectionMedium:BasalMediumwith200µg/mLhygromycinBand400ug/mLG418.
  3. ThawingandSeedingCells
    1. PrepareBasalMedium.Prepare37°CWaterBath.Thawcellsrapidlybyremovingfromliquidnitrogen,andimmediatelyimmersingina37°Cwaterbath.Removethevialfromthewaterbathassoonasthecontentsarethawed,andsterilizetheexteriorofthevialwith70%ethanol.Alloftheoperationsfromthispointonshouldbecarriedoutunderstrictasepticconditions.
    2. Addvialcontentsto25mLcellculturemediuminT75TissueCultureTreatedFlask.Gentlyswirlflaskandplaceinahumidified,tissuecultureincubator,37°C,5%CO2.
    3. Alllivecellsshouldbeattachedafter18-24hourspost–thaw.ChangeBasalMediumwithSelectionMedium.
  4. SubcultureandPropagation
    1. Whencellsareapproximately80%confluent,passagethecells.
    2. Passagethecells1:10every3-4days,usingTrypsin-EDTAtodissociatethecells.
    3. Itishighlyrecommendedthatafrozencellbankbeestablishedatlowpassagenumber.
References&Citations
CitationExplorer

ActivationofP2X7andP2Y11purinergicreceptorsinhibitsmigrationandnormalizestumor-derivedendothelialcellsviacAMPsignaling
Authors:DAvanzato,TGenova,AFiorioPla,MBernardini,SBianco,BBussolati,DMancardi,EGiraudo,FMaione,PCassoni
Journal:ScientificReports(2016)

TheM2muscarinicreceptorsareessentialforsignalingintheheartleftventricleduringrestraintstressinmice
Authors:HanaTomankova,PaulinaValuskova,EvaVarejkova,JanaRotkova,JanBenes,JaromirMyslivecek
Journal:Stress(2015)


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
联络我们
服务热线:4000-520-616
(限工作日9:00-18:00)
QQ :1570468124
手机:18915418616